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Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

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Updated: Jun 16, 2026

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
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Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

Satellite cell characterization from aging human muscle.

Arianna Corbu1, Annarita Scaramozza, Lucilla Badiali-DeGiorgi

  • 1Clinical Department of Radiological and Histopathological Sciences, University of Bologna, Bologna, Italy.

Neurological Research
|January 23, 2010
PubMed
Summary
This summary is machine-generated.

Aging human satellite cells (SCs) can still form muscle fibers, but their regenerative potential is compromised in vitro. This study investigated SC proliferation and differentiation in older individuals.

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Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
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Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Published on: August 23, 2019

Area of Science:

  • Muscle biology and regeneration
  • Cellular aging and senescence
  • Skeletal muscle progenitor cells

Background:

  • Satellite cells (SCs) are crucial for muscle growth and repair.
  • Aging in humans leads to SC depletion and reduced proliferation, but functional capacity remains unclear.
  • The impact of advanced age on SC response to muscle injury and denervation is not well understood.

Purpose of the Study:

  • To investigate muscle regeneration by analyzing the proliferative and differentiation capacity of SCs in aging human patients.
  • To deeply study the molecular mechanisms underlying the aging of SCs.

Main Methods:

  • Isolation of SCs from aging human muscle biopsies.
  • Morphological analysis using transmission electron microscopy and immunocytochemistry (desmin, N-CAM, M-cadherin).
  • In vitro culture to assess growth, expansion, and myogenic gene expression (Myf5, MyoD, myogenin) via RT-PCR.

Main Results:

  • SCs from aging muscle biopsies successfully underwent myogenic program and formed myotubes in vitro, albeit at a slower rate than young controls.
  • Ultrastructural analysis revealed compromised SC features.
  • RT-PCR indicated a potential compromise in myogenic potential during in vitro proliferation of aged SCs.

Conclusions:

  • Despite challenges, aging human SCs retain the ability to proliferate and differentiate into myotubes under favorable conditions.
  • The study suggests that the intrinsic myogenic potential of SCs may be compromised with advanced age.
  • Further research is needed to fully elucidate the aging mechanisms affecting SC function in vivo.