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Neurulation

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...

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Related Experiment Video

Updated: Jun 16, 2026

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Nogo-a regulates neural precursor migration in the embryonic mouse cortex.

Carole Mathis¹, Aileen Schröter, Michaela Thallmair

¹Brain Research Institute, University of Zurich and Department of Biology, ETH Zurich, 8057 Zurich, Switzerland.

Cerebral Cortex (New York, N.Y. : 1991)

|January 23, 2010

Summary

Nogo-A regulates neuronal precursor migration in the developing brain. Knocking out Nogo-A enhances cell motility and disrupts normal cortical development, revealing its crucial role in brain formation.

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Last Updated: Jun 16, 2026

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Published on: March 8, 2024

Area of Science:

Neuroscience
Developmental Biology
Cell Biology

Background:

Nogo-A is known for inhibiting axonal regeneration in the adult central nervous system.
Its function during embryonic brain development remains largely uncharacterized.
Nogo-A is expressed in radial glial cells and migrating neurons in the embryonic mouse cortex.

Purpose of the Study:

To investigate the role of Nogo-A in the radial migration of neuroblasts during embryonic cortical development.
To determine the effect of Nogo-A and its receptor complex on neuronal precursor motility and migration.

Main Methods:

Studied radial migration of neuroblasts in wild-type and Nogo-A knockout (KO) mouse embryos.
Utilized in vitro analysis of cells from embryonic neurospheres.
Performed live imaging to assess cell motility.
Employed Bromodeoxyuridine (BrdU) labeling to track neuronal precursor migration patterns.

Main Results:

Nogo-A and its receptor components (NgR, Lingo-1, TROY, p75) are expressed in emigrating neuroblasts.
Nogo-A knockout or blockade significantly increased cell motility.
Blocking NgR or Lingo-1 also enhanced cell motility, indicating a direct role for surface Nogo-A.
Nogo-A influences radial migration, with KO embryos showing disrupted precursor accumulation and migration to upper cortical layers.

Conclusions:

Nogo-A and its receptor complex are critical regulators of radial neuronal migration during cortical development.
Nogo-A plays a role in balancing adhesive and repulsive cell interactions that guide migrating neurons.
These findings highlight a novel function of Nogo-A in the developing central nervous system.