Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Deconvolution01:20

Deconvolution

Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Reconstruction of Signal using Interpolation01:10

Reconstruction of Signal using Interpolation

Signal processing techniques are essential for accurately converting continuous signals to digital formats and vice versa. When a continuous signal is sampled with a period T, the resulting sampled signal exhibits replicas of the original spectrum in the frequency domain, spaced at intervals equal to the sampling frequency. To handle this sampled signal, a zero-order hold method can be applied, which creates a piecewise constant signal by retaining each sample's value until the next sampling...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Meralgia paraesthetica.

BJA education·2025
Same author

Determining sample size for progression criteria for pragmatic pilot RCTs: the hypothesis test strikes back!

Pilot and feasibility studies·2021
Same author

The cytoskeletal motor proteins Dynein and MyoV direct apical transport of Crumbs.

Developmental biology·2019
Same author

Forces shaping the Drosophila wing.

Mechanisms of development·2016
Same author

Sex comparisons of strength and coactivation following ten weeks of deadlift training.

Journal of musculoskeletal & neuronal interactions·2014
Same author

The effects of a high-intensity free-weight back-squat exercise protocol on postural stability in resistance-trained males.

Journal of sports sciences·2014
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Holographic subtraction.

K Bromley, M A Monahan, J F Bryant

    Applied Optics
    |January 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a holographic subtraction technique for comparing optical fields. It enables precise change detection by isolating differences between two dissimilar optical fields using interference.

    More Related Videos

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
    07:27

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

    Published on: November 1, 2017

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
    05:45

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

    Published on: March 31, 2022

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

    Published on: February 8, 2014

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
    07:27

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

    Published on: November 1, 2017

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
    05:45

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

    Published on: March 31, 2022

    Area of Science:

    • Optics and Photonics
    • Holography
    • Optical Engineering

    Background:

    • Holography enables the recording and reconstruction of wavefronts.
    • Comparing optical fields often requires specialized techniques for precise analysis.
    • Existing methods may lack the simplicity or accuracy for certain applications.

    Purpose of the Study:

    • To present a novel holographic subtraction technique.
    • To enable the isolation of differences between two dissimilar optical fields.
    • To demonstrate the technique's applicability in change detection.

    Main Methods:

    • Utilizing optical interference between a holographically reconstructed field and a direct real-time field.
    • Applying the principle to image-plane, Fourier-transform, and Fresnel side-band holograms.
    • Experimental verification of the holographic subtraction method.

    Main Results:

    • Successful subtraction of two dissimilar optical fields to yield their difference.
    • Demonstrated feasibility across various holographic types (image-plane, Fourier-transform, Fresnel).
    • Effective application for change detection between time-varying transparencies.

    Conclusions:

    • The presented holographic subtraction technique is effective for isolating optical field differences.
    • The method offers a viable approach for change detection applications.
    • This technique advances holographic capabilities for comparative optical analysis.