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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...

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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

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Published on: May 18, 2011

An interferometric rejection filter for Raman spectroscopy.

W Proffitt, L M Fraas, P Cervenka

    Applied Optics
    |January 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel three-mirror interferometer acts as a powerful rejection filter for Raman scattering experiments. This device effectively blocks laser light while preserving the desired Raman signal, improving experimental accuracy.

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    Area of Science:

    • Optics and Photonics
    • Spectroscopy
    • Laser Technology

    Background:

    • Raman scattering experiments require effective suppression of elastically scattered laser light.
    • Traditional filters often struggle to achieve sufficient attenuation without compromising signal detection.

    Purpose of the Study:

    • To describe and analyze a three-mirror, multiple beam interferometer for use as a rejection filter.
    • To demonstrate the filter's performance in attenuating laser source frequency light and passing Raman scattered signal.

    Main Methods:

    • Design and theoretical analysis of a three-mirror, multiple beam interferometer.
    • Experimental implementation and testing of a prototype filter in a Raman scattering setup.

    Main Results:

    • The prototype filter achieved an attenuation factor of 450 for laser source frequency light.
    • The filter successfully passed 88% of the Raman scattered signal.

    Conclusions:

    • The developed three-mirror interferometer serves as an effective rejection filter for Raman spectroscopy.
    • This technology offers significant improvements in signal-to-noise ratio for Raman scattering measurements.