Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...

UV–Vis Spectroscopy: Molecular Electronic Transitions

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...

UV–Vis Spectroscopy: Beer–Lambert Law

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...

UV–Vis Spectrum

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...

UV–Vis Spectroscopy of Conjugated Systems

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π&VeryThinSpace;→&VeryThinSpace;π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rocket-borne instrument with a high-resolution microchannel plate detector for planetary UV spectroscopy.

Applied optics·2010

Same author

Selective infrared gas analyzers.

Journal of the Optical Society of America·2010

Same author

Rocket instrument for far-UV spectrophotometry of faint astronomical objects.

Applied optics·2010

Same author

Construction, calibration, and application of a compact spectrophotometer for EUV (300-2500-A) plasma diagnostics.

Applied optics·2010

Same author

Comprehensive analysis of gratings for ultraviolet space instrumentation.

Applied optics·2010

Same author

Compact far ultraviolet emission source with rich spectral emission 1150-3100 A.

Applied optics·2010

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

Mariner 6 and 7 ultraviolet spectrometers.

J B Pearce, K A Gause, E F Mackey

|January 23, 2010

Summary

This summary is machine-generated.

Ultraviolet spectrometers captured Mars' upper atmosphere emission spectrum in 1969. The instruments successfully measured wavelengths from 1100-4300 A, providing valuable atmospheric data.

More Related Videos

Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

Published on: September 14, 2016

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Published on: October 30, 2012

Related Experiment Videos

Last Updated: Jun 16, 2026

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

Published on: September 14, 2016

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Published on: October 30, 2012

Area of Science:

Planetary Science
Atmospheric Science
Spectroscopy

Background:

Early space missions provided opportunities for in-situ atmospheric analysis.
Understanding Mars' atmospheric composition is crucial for planetary evolution studies.

Purpose of the Study:

To measure the ultraviolet emission spectrum of the Martian upper atmosphere.
To assess the performance of specialized ultraviolet spectrometers in a planetary environment.

Main Methods:

Utilized a planetary coronagraph and an Ebert-Fastie monochromator for ultraviolet observations.
Employed photomultiplier tubes with cesium iodide and bialkali photocathodes to detect spectral ranges from 1100-4300 A.
Calibrated instruments using a tungsten lamp, sodium salicylate screen, and nitric oxide cell.

Main Results:

Successfully rejected off-axis light interference at distances up to 6600 km.
Measured the emission spectrum of Mars' upper atmosphere at altitudes as low as 170 km.
Acquired spectral data in the 1100-4300 A range.

Conclusions:

The ultraviolet spectrometers were effective for Martian atmospheric analysis.
The study provided early spectral data of Mars' upper atmosphere.
Demonstrated the capability of instruments to operate and collect data in interplanetary space.