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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
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Rapid scanning microscope for light probing and infrared mapping.

R J Phelan, N L Demeo

    Applied Optics
    |January 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel rapid scanning microscope uses a vibrating single mirror to create a raster pattern for light-probing semiconductor devices. This system efficiently observes infrared emission and transmission with a wide field of view.

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    Area of Science:

    • Optoelectronics
    • Materials Science
    • Microscopy

    Background:

    • Semiconductor device analysis requires advanced imaging techniques.
    • Observing infrared emission and transmission is crucial for device characterization.

    Purpose of the Study:

    • To design and implement a rapid scanning microscope for semiconductor device analysis.
    • To enable efficient light-probing and infrared observation of devices.

    Main Methods:

    • Development of a rapid scanning microscope centered around a single, dual-axis vibrating mirror.
    • Utilizing standard microscope components for system integration.
    • Deflecting a light beam into a raster pattern for wide-angle scanning.

    Main Results:

    • Successful demonstration of the microscope for light-probing semiconductor devices.
    • Effective observation of infrared emission and transmission from devices.
    • Achieved useful wide-angle deflections and a large field of view.

    Conclusions:

    • The single mirror scanner is a simple yet effective component for rapid microscopy.
    • The developed system offers a practical approach to semiconductor device characterization.
    • The microscope design allows for versatile applications in optoelectronics and materials science.