Related Experiment Video
Updated: Jun 16, 2026

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
Chemical structure of Bacteriovorax stolpii lipid A
Sebastian Beck1, Frederic D Müller, Eckhard Strauch
1Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany.
Abstract:
Bdellovibrionales is a phylogenetically diverse group of predatory prokaryotes, which consists of the two families Bdellovibrionaceae and Bacteriovoracaceae. We describe LPS and lipid A of the type strain Bacteriovorax stolpii DSM 12778, representing the first characterized endotoxin of a Bacteriovoracaceae member. It has a smooth form LPS, which was identified by SDS-polyacrylamide gel electrophoresis. The lipid A structure was determined by combined gas chromatography-mass spectrometry, electrospray ionization mass spectrometry and NMR spectroscopy. Its backbone consists of two beta-(1-->6)-linked 2,3-diamino-2,3-dideoxy-D-glucopyranoses (GlcpN3N) carrying a pyrophosphoethanolamine at O-4' of the non-reducing sugar and a phosphate group linked to O-1 of the reducing GlcpN3N. Positions 2, 3, 2' and 3' of the two GlcpN3N are acylated with primary 3-hydroxy fatty acids and one of those carries a secondary fatty acid.
More Related Videos
Related Concept Videos
Formation of Lipopolysaccharides
Structure of Lipids
Structure of Lipids
Structure of Lipids
Viral Structure
What are Lipids?
Non-Polar and Hydrophobic Characteristics of Lipids
Lipids are a structurally and functionally diverse group of hydrocarbons—compounds consisting of carbon and hydrogen atoms. The carbon-carbon and carbon-hydrogen bonds...

