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Updated: Jun 16, 2026
Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model
Published on: May 21, 2013
[Age-related biomechanical properties of chondrocytes in rabbit knee articular cartilage]
Xiao-Chun Wei1, Chun-Jiang Li, Quan-You Zhang
1Department of Orthopedics, Second Hospital of Shanxi Medical Univeristy, Taiyuan 030001, China. weixiaochun06@yahoo.com.cn
Objective:
To fully demonstrate the alterations about the viscoelastic properties of chondrocytes in rabbit knee articular cartilage.
Methods:
Fifteen New Zealand white rabbits were divided into 3 age groups: young group (1 month), adult group (8 months) and old group (31 months). All rabbits were sacrificed and isolated from knee joint and digested into chondrocytes. The micropipette aspiration combined with Half-space model was used to quantify changes in viscoelastic properties of chondrocytes.
Results:
Experimental studies have shown that the changes of aspiration length with time in old group were obviously different from young group and adult group. But similar variations were found between the latter two groups. The viscoelastic properties of chondrocytes in old group exhibited a significantly lower instantaneous modulus E0 (0.55 +/- 0.05 kPa), equilibrium modulus E infinity (0.28 +/- 0.04 kPa) and apparent viscosity micro (4.10 +/- 0.61 kPa x s) as compared with young group (0.67 +/- 0.10), (0.37 +/- 0.09), (6.29 +/- 0.92) kPa x s (P < 0.001) versus adult group: (0.65 +/- 0.07), (0.35 +/- 0.05), (6.01 +/- 0.89) kPa x s (P < 0.001). But no difference were found between the latter two groups (P > 0.05).
Conclusion:
In response to a step pressure, chondrocytes in each group exhibited the viscoelastic solid creep behavior. The viscoelastic properties of chondrocytes markedly decreased in old group.
