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The bactericidal effect of a Genius Nd:YAG laser
Aa Kranendonk1, Wa van der Reijden, Aj van Winkelhoff
1Department of Periodontology, Academic Centre for Dentistry Amsterdam, Amsterdam, The Netherlands.
Purpose:
To evaluate the 'in vitro' bactericidal effect of the Nd:YAG laser (Genius, MØlsgaard Dental, Copenhagen, Denmark) on six periodontal pathogens.
Methods:
Suspensions of six different periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum and Parvimonas micra) were prepared in small Eppendorff tubes, and exposed to a Nd:YAG laser for five different periods of time. Laser settings used: Power 6 Watt (on a scale of 1-12 W), Frequency 50 Hz, Pulse duration 250 mus. After exposure to the laser, aliquots of the suspensions were spread on blood agar plates for bacterial counting.
Results:
After 5 s of laser exposure, there was a decrease in total colony forming units for all six selected microorganisms. After 15, 30 and 45 s, no viable bacterial cells could be retrieved.
Conclusion:
In this 'in vitro' model, 15 s of Nd:YAG laser use was found to be effective for total killing of the six tested periodontal pathogens.
