Related Experiment Video
Updated: Jun 16, 2026
09:41
Performing and Processing FNA of Anterior Fat Pad for Amyloid
Published on: October 30, 2010
[Systemic amyloidosis: practical diagnosis]
1Inserm U 845, hôpital Necker, 161, rue de Sèvres, 75015 Paris, France. laure-helene.noel@inserm.fr
Nephrologie & Therapeutique
|January 26, 2010
Summary
This study details amyloid deposit characteristics, including ultrastructural appearance and Congo red staining. It guides renal pathologists on diagnostic tissues, staining methods, and immunohistochemical characterization for accurate amyloidosis diagnosis.
Area of Science:
- Pathology
- Biochemistry
- Histology
Context:
- Amyloid deposits are characterized by specific ultrastructural features, including 7-10 nm fibrils.
- Amyloid identification relies on tinctorial properties like Congo red positivity, exhibiting apple-green birefringence under polarized light.
- Immunohistochemistry on frozen tissue is crucial for determining the origin of amyloidosis.
Purpose:
- To provide renal pathologists with a guide to studying amyloidosis.
- To highlight potential diagnostic challenges and pitfalls.
- To outline optimal tissues, staining techniques, and immunohistochemical characterization methods.
Summary:
- Amyloid deposits possess distinct ultrastructural morphology (7-10 nm fibrils) and tinctorial properties (Congo red positivity with apple-green birefringence).
- Accurate diagnosis of amyloidosis requires immunohistochemical characterization on frozen tissue to identify the amyloid origin.
- This guidance aims to improve diagnostic accuracy by clarifying tissue selection, staining, and characterization protocols.
Impact:
- Enhances diagnostic accuracy for amyloidosis in renal pathology.
- Provides a standardized approach to amyloidosis characterization.
- Aids in understanding the origin and implications of amyloid deposition in kidney disease.
More Related Videos
Related Concept Videos
01:27Atherosclerosis II: Clinical Manifestations and Diagnostic Tests
Atherosclerosis is a progressive disorder that leads to the thickening and narrowing of arterial walls due to plaque buildup. This condition can cause various symptoms depending on the arteries affected:Coronary Artery Disease (CAD): This condition affects the coronary arteries and may lead to chest pain (angina), shortness of breath (dyspnea), heart attacks, and other heart disease symptoms.Cerebrovascular Disease: This affects blood flow to the brain, causing transient ischemic attacks (TIAs)...
03:03Amyloid Fibrils
Amyloid fibrils are aggregates of misfolded proteins. Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils.
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
01:27Myocarditis II: Clinical Features and Diagnostic Tests
Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...