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Updated: Jun 16, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

Isorhamnetin-induced anti-adipogenesis is mediated by stabilization of beta-catenin protein

Jongsung Lee1, Jienny Lee, Eunsun Jung

  • 1Biospectrum Life Science Institute, 101-701 SK Ventium, 522 Dangjung Dong, Gunpo City, 435-833 Gyunggi Do, Republic of Korea.

Life Sciences
|January 26, 2010
PubMed
Abstract

Insights

Isorhamnetin inhibits human stem cell differentiation into fat cells by stabilizing beta-catenin. This natural compound

Area of Science:

  • Biochemistry
  • Cell Biology
  • Stem Cell Research

Background:

  • Isorhamnetin is known to possess anti-adipogenic properties.
  • Understanding its mechanism in human cells is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the inhibitory mechanisms of isorhamnetin on adipogenic differentiation in human adipose tissue-derived stem cells (hAMSCs).

Main Methods:

  • Quantified adipogenic differentiation using Oil Red O staining and triglyceride assays.
  • Analyzed adipogenesis-related gene expression via real-time PCR and Western blot.

Main Results:

  • Isorhamnetin inhibited hAMSC adipogenesis.
  • Down-regulated Wnt antagonists (sFRP1, Dkk1) and Wnt receptor/co-receptor genes.
  • Increased beta-catenin protein levels and GSK 3beta phosphorylation.
  • Upregulated beta-catenin target genes (c-myc, cyclin D1, PPARdelta) and downregulated key adipogenic factors (C/EBPalpha, PPARgamma).

Conclusions:

  • Isorhamnetin effectively inhibits adipogenic differentiation in hAMSCs.
  • The mechanism involves the stabilization of beta-catenin, a key Wnt signaling effector.

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