Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Transport to the Stroma01:24

Protein Transport to the Stroma

Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein Transport to the Outer Chloroplast Membrane01:11

Protein Transport to the Outer Chloroplast Membrane

Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Protein Transport to the Inner Chloroplast Membrane01:18

Protein Transport to the Inner Chloroplast Membrane

Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
Morphogenesis02:19

Morphogenesis

Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Performance Weavable Piezoelectret Fibers via Scalable In Situ Poling Melt-Spinning for Real-Time Knee Joint Rehabilitation Monitoring.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

A CD36-targeted aptamer-4-butyl-polyhydroxybenzophenone conjugate with pH-responsive release for liver delivery in MASLD.

Regenerative biomaterials·2026
Same author

Ultrasensitive Hydrogen Sensor Based on a Non-Stoichiometric Tin Oxide Heterojunction for LIB Thermal Runaway Early Monitoring.

ACS sensors·2026
Same author

Scion varieties and nitrogen levels affect carbon and nitrogen assimilation in apple via modulating rhizosphere microbial structure and function.

Horticulture research·2026
Same author

Improving low-phosphate tolerance via tissue-specific CRISPR/Cas9 knockout to balance growth and stress responses in rice.

The Plant cell·2026
Same author

Accelerating Water Dissociation by Interfacial Conductance Clamp.

ChemSusChem·2026

Related Experiment Video

Updated: Jun 16, 2026

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
05:22

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring

Published on: January 20, 2023

A rice stromal processing peptidase regulates chloroplast and root development.

Runqing Yue1, Xiaofei Wang, Jieyu Chen

  • 1State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, PR China.

Plant & Cell Physiology
|January 26, 2010
PubMed
Summary

Stromal processing peptidase (SPP) is crucial for rice chloroplast development and root growth. A rice mutant lacking functional SPP exhibited chlorosis and inhibited root development, highlighting SPP's essential role.

More Related Videos

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Related Experiment Videos

Last Updated: Jun 16, 2026

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
05:22

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring

Published on: January 20, 2023

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Stromal processing peptidase (SPP) is a metalloendopeptidase known to process precursor substrates.
  • Its specific functions in monocot plants, particularly rice, remain largely uncharacterized.

Purpose of the Study:

  • To investigate the function of SPP in rice.
  • To elucidate the role of SPP in plant development and chloroplast biogenesis.

Main Methods:

  • Isolation and genetic analysis of a rice mutant exhibiting leaf chlorosis and root growth inhibition.
  • Map-based cloning to identify the mutated gene encoding SPP.
  • Transgenic complementation, subcellular localization, Western blot analysis, and gene expression analysis.

Main Results:

  • A rice mutant with a glutamate deletion in SPP displayed early seedling leaf chlorosis and persistent root growth inhibition.
  • SPP is localized to the chloroplast and is essential for chloroplast development and Rubisco small subunit processing in early growth stages.
  • SPP plays a vital role in rice root development throughout the entire growth period.

Conclusions:

  • SPP is essential for chloroplast biogenesis during early rice growth stages.
  • SPP is critical for rice root development.
  • This study provides the first report on the function of SPP in monocot plants.