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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

[Double strand break of DNA in gastric adenoma and adenocarcinoma]

Jeong Ho Kim1, Sung Soo Kim, Sang Won Byun

  • 1Department of Internal Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea. jkkim488@yahoo.co.kr

The Korean Journal of Gastroenterology = Taehan Sohwagi Hakhoe Chi
|January 26, 2010
PubMed
Abstract

Insights

DNA double-strand breaks (DSBs) are linked to gastric cancer development. Researchers found elevated 53BP1 and gamma-H2AX protein expression in gastric adenocarcinoma, but not in premalignant adenoma tissues.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • DNA double-strand breaks (DSBs) are critical DNA damage. Unrepaired DSBs can lead to chromosomal instability and tumorogenesis, especially in p53-mutated cells.
  • DSBs have been observed in malignant cancers and premalignant lesions.
  • Gastric cancer and its precursor lesions are significant health concerns globally.

Purpose of the Study:

  • To investigate the differential expression of 53BP1 and gamma-H2AX, established markers for DSBs.
  • To compare the expression levels of these DSB markers in normal gastric tissues, gastric adenomas, and gastric adenocarcinomas.

Main Methods:

  • Tissue microarray construction using samples from 121 gastric adenocarcinoma and 51 gastric adenoma patients.
  • Immunohistochemical staining for 53BP1 and gamma-H2AX in tissue microarrays.
  • Inclusion of normal gastric tissues from gastric adenocarcinoma patients for comparison.

Main Results:

  • Significantly higher expression of 53BP1 and gamma-H2AX was observed in gastric carcinoma cells compared to normal and adenoma tissues (p<0.01).
  • No significant difference in 53BP1 and gamma-H2AX expression was found between normal epithelium and gastric adenoma.
  • Elevated 53BP1 expression correlated with higher atypia grades (II and III) in adenomas.

Conclusions:

  • DNA double-strand breaks appear to be associated with the progression to gastric adenocarcinoma.
  • DSBs do not seem to significantly impact premalignant adenoma cells.
  • These findings suggest DSB markers could play a role in understanding gastric carcinogenesis.

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