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Updated: Jun 16, 2026
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
Published on: February 27, 2014
[Saccharomyces boulardii reduced intestinal inflammation in mice model of 2,4,6-trinitrobencene sulfonic acid induced
Sang Kil Lee1, Hyo Jong Kim, Sung Gil Chi
1Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. sklee@yuhs.ac
Background/Aims:
Saccharomyces boulardii has been reported to be beneficial in the treatment of inflammatory bowel disease. The aim of this work was to evaluate the effect of S. boulardii in a mice model of 2,4,6-trinitrobencene sulfonic acid (TNBS) induced colitis and analyze the expression of genes in S. boulardii treated mice by microarray.
Methods:
BALB/c mice received TNBS or TNBS and S. boulardii treatment for 4 days. Microarray was performed on total mRNA form colon, and histologic evaluation was also performed.
Results:
In mice treated with S. boulardii, the histological appearance and mortality rate were significantly restored compared with rats receiving only TNBS. Among 330 genes which were altered by both S. boulardii and TNBS (>2 folds), 193 genes were down-regulated by S. boulardii in microarray. Most of genes which were down-regulated by S. bouardii were functionally classified as inflammatory and immune response related genes.
Conclusions:
S. boulardii may reduce colonic inflammation along with regulation of inflammatory and immune responsive genes in TNBS-induced colitis.
Insights
Saccharomyces boulardii treatment improved histological appearance and survival in a mouse model of inflammatory bowel disease. This probiotic down-regulated inflammatory and immune response genes in the colon.
Area of Science:
- Gastroenterology
- Microbiology
- Immunology
Context:
- Inflammatory bowel disease (IBD) poses a significant health challenge.
- The probiotic Saccharomyces boulardii (S. boulardii) has shown potential therapeutic benefits in IBD.
- A murine model of 2,4,6-trinitrobencene sulfonic acid (TNBS)-induced colitis was utilized to investigate S. boulardii's effects.
Purpose:
- To evaluate the therapeutic efficacy of S. boulardii in a TNBS-induced colitis mouse model.
- To analyze the impact of S. boulardii on gene expression related to inflammation and immune response in the colon.
Summary:
- S. boulardii treatment significantly improved histological outcomes and reduced mortality rates in mice with TNBS-induced colitis.
- Microarray analysis revealed that S. boulardii down-regulated 193 out of 330 genes affected by TNBS.
- The majority of down-regulated genes were associated with inflammatory and immune responses.
Impact:
- S. boulardii demonstrates a protective effect against chemically induced colitis in mice.
- The probiotic modulates the colonic inflammatory and immune gene expression profile.
- Findings suggest S. boulardii as a potential therapeutic agent for managing inflammatory bowel conditions.
