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Related Concept Videos

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Related Experiment Video

Updated: Jun 16, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Highly sensitive protein detection using enzyme-labeled gold nanoparticle probes.

Meiying Liu1, Chunping Jia, Yunyan Huang

  • 1Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai, 200050, P.R. China.

The Analyst
|January 26, 2010
PubMed
Summary

A novel enzyme-labeled gold nanoparticle probe offers highly sensitive protein detection. This new immunoassay method significantly outperforms traditional enzyme-linked immunosorbent assay (ELISA) for detecting biomarkers like carcinoembryonic antigen (CEA).

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Last Updated: Jun 16, 2026

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Protein detection is crucial for disease diagnosis.
  • Existing methods like ELISA have limitations in sensitivity and speed.
  • Novel nanomaterial-based probes can enhance immunoassay performance.

Purpose of the Study:

  • To develop a highly sensitive protein detection method using enzyme-labeled gold nanoparticle (AuNP) probes.
  • To evaluate the performance of this novel immunoassay for detecting carcinoembryonic antigen (CEA).
  • To demonstrate the potential application of the method in clinical diagnostics.

Main Methods:

  • Preparation of an enzyme-labeled AuNP probe coated with antibody, single-stranded DNA (ssDNA), and horseradish peroxidase (HRP).
  • Utilizing magnetic microparticles (MMP) functionalized with antibodies as capture probes.
  • Employing a sandwich immunoreaction followed by signal transduction via enzymatically amplified optical signal.

Main Results:

  • The developed method achieved a detection limit of 12 ng L(-1) for CEA.
  • This sensitivity is approximately 130-fold higher than conventional ELISA.
  • The immunoassay demonstrated high sensitivity and good specificity in detecting CEA in serum samples.

Conclusions:

  • The novel enzyme-labeled AuNP probe immunoassay provides a highly sensitive and specific method for protein detection.
  • This approach offers significant advantages over conventional ELISA.
  • The method shows promising potential for early disease diagnosis through biomarker detection.