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Updated: Jun 16, 2026

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
[Antifungal effect of high molecular weight chitosan on Candida spp isolated from clinical samples]
Cecilia P Tapia1, Diego M Soto, Leonardo G Vergara
1Programa de Microbiología y Micologia, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile. cetapia@med.uchile.cl
Unlabelled:
Chitosan is a D-glucosamine polysaccharide derived from chitin that displays an antimicrobial activity against bacteria and fungi.
Objective:
to evaluate the antifungal effect of high molecular weight chitosan (HMWC) in clinical strains of Candida spp.
Methodology:
the susceptibility of forty strains of Candida spp. to HMWC was studied (16 C albicans, 11 C glabrata, 5 C. tropicalis, 5 C krusei, 2 C parapsilosis and 2 C. famata) by broth microdilution at pH 7.0 and pH 4.0.
Results:
of 40 strains, only 2 were inhibited at pH 7.0 and corresponded to ATCC control strains (C. krusei 6258 and C parapsilosis 22019). On the other hand, 37/40 strains (92.5%) were inhibited by concentrations lower than 1.25 mg/mL of HMWC at pH 4.0.
Conclusion:
these results show that HMWC, presents activity against clinical Candida spp. strains, including C glabrata, and that this activity is present at acid pH (4.0). This compound could potentially be used in vulvovaginal candidiasis since it occurs at pH 4.0-4.5.
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