Related Experiment Video
Updated: Jun 16, 2026

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
Published on: June 9, 2015
Micropropagation of Codiaeum variegatum (L.) Blume and regeneration induction via adventitious buds and somatic
1Centro de Estudios Farmacológicos y Botánicos (UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
Abstract:
Codiaeum variegatum (L) Blume cv. "Corazon de oro" and cv. "Norma" are successfully micropropagated when culture are initiated with explants taken from newly sprouted shoots. The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, according to the cultivar respectively selected.Adventive organogenesis and somatic embryogenesis are induced from leaf explants taken from in vitro buds of croton. On leaf-sectioned of "Corazon de oro" cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development and induces some shoots too. Replacing BA with 1 mg/L TDZ induces up to 100% bud regeneration in the same explants. On the other hand, leaf-sectioned of C. variegatum cv. Norma does not start somatic embryo differentiation if 1 mg/L TDZ is not added to the MS basal medium. Incipient callus is observed after 30 days of culture, and then, subculture to MS with 1 mg/L BA allows the same process to show on the "Corazon de oro" cultivar. Somatic embryos show growth arrest that is partially overcome by transfer to hormone-free basal medium with activated charcoal. Root induction is possible on basal medium plus 1 mg/L IBA. Plantlets in the greenhouse have variegated leaves true-to-type.
More Related Videos
07:19Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds
Published on: September 19, 2025
06:30Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica
Published on: January 16, 2026
Related Concept Videos
Asexual Reproduction
Plant Tissue Culture
Seed Structure and Early Development of the Sporophyte
Primary and Secondary Growth in Roots and Shoots
Overview of Regeneration and Repair
Regeneration
All animals have varying degrees of...
Whole Body Regeneration