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Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis
Published on: May 12, 2023
Adjuvant effects on antibody titre.
1National Institute for Biological Standards and Controls, Potters Bar, Hertfordshire, UK. Barry.Walker@nibsc.hpa.org.uk
Methods in Molecular Biology (Clifton, N.J.)
|January 26, 2010
Summary
Antibody titre quantifies specific antibodies in blood, commonly measured using enzyme-linked immunosorbent assay (ELISA). This robust method is adaptable for quality control and detailed in a versatile ELISA protocol.
Area of Science:
- Biochemistry
- Immunology
Background:
- Antibody titre measures specific antibodies in blood.
- Enzyme-linked immunosorbent assay (ELISA) is a key technique for determining antibody concentration.
Purpose of the Study:
- To detail a specific ELISA protocol.
- To present a versatile ELISA method with potential for broad application.
Main Methods:
- Utilized enzyme-linked immunosorbent assay (ELISA) technology.
- Followed a specific, detailed ELISA protocol.
Main Results:
- Established a method for quantifying antibody concentration.
- Demonstrated the robustness and adaptability of the ELISA technique.
Conclusions:
- The described ELISA protocol offers a reliable method for antibody titre determination.
- ELISA is a robust, quality-controllable technique suitable for various applications.
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