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Adjuvant effects on antibody titre

Barry Walker1, Ian Feavers

  • 1National Institute for Biological Standards and Controls, Potters Bar, Hertfordshire, UK. Barry.Walker@nibsc.hpa.org.uk

Methods in Molecular Biology (Clifton, N.J.)
|January 26, 2010
PubMed

Antibody titre is a measure of the presence and amount of antibodies specific to an antigen that are present in the blood. In particular, the titre of an antibody sample is a measure of the antibody concentration determined under a defined set of conditions, with the antibody concentration being commonly established by enzyme-linked immunosorbent assay, also called ELISA, or a variation of this technology.Enzyme-linked immunosorbent assay, also called ELISA, enzyme immunoassay or EIA, is a biochemical technique used to detect the presence of an antibody or an antigen in a sample. The ELISA/EIA approach is an extremely robust technology and readily amenable to validation and quality control if adherence to ISO quality standards is required. This chapter describes in detail a specific ELISA protocol which has potential generic application.

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