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Related Experiment Video

Updated: Jun 16, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

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Published on: August 16, 2019

Bioaffinity chromatography on monolithic supports.

Kishore K R Tetala¹, Teris A van Beek

¹Laboratory of Organic Chemistry, Natural Products Chemistry Group, Wageningen University, Wageningen, The Netherlands. kishore.tetala@gmail.com

Journal of Separation Science

|January 26, 2010

Summary

This review highlights monolithic materials for bioaffinity chromatography, enabling rapid analysis and biomarker enrichment. Recent research showcases diverse ligands and applications, with epoxy CIM disks being the most utilized format.

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Area of Science:

Analytical Chemistry
Biochemistry

Background:

Affinity chromatography on monolithic supports offers fast analyses, small sample volumes, and strong enrichment of trace biomarkers.
Monolithic materials are increasingly used in bioaffinity chromatography, including immunochromatography, for analytical and preparative applications.

Purpose of the Study:

To review recent research on monolithic materials in bioaffinity chromatography.
To discuss biomolecules used as ligands, monolith preparation, formats, and applications.

Main Methods:

Review of recent scientific literature (37 papers in the last four years).
Discussion of various biomolecules (antibodies, enzymes, lectins, aptamers) as ligands.
Exploration of monolith preparation, formats (disks, capillaries, microchips), and ligand immobilization.

Main Results:

Protein A and G remain primary ligands for immunoglobulin enrichment.
Antibodies and lectins are popular for analyzing smaller molecules and saccharides.
Cryogels with ligands are used for cell and bacteria sorting; aptamers and phages are new ligand applications.
Convective interaction media (epoxy CIM disks) are the most common format.

Conclusions:

Monolithic bioaffinity chromatography is a powerful analytical platform with diverse and evolving applications.
The choice of ligand and monolith format depends on the target analyte, with established ligands still dominant but new ones emerging.