Related Experiment Video
Updated: Jun 16, 2026

Impact of Common Fruit Juices and Toothbrushing on the Discoloration of Primary Teeth
Published on: March 27, 2026
Oral fluoride retention in orthodontic patients with and without fixed appliances after using different fluoridated
Hosam Baeshen1, Heidrun Kjellberg, Dowen Birkhed
1Department of Orthodontics, Institute of Odontology, The Sahlgrenska Academy at the University of Gothenburg, Sweden.
Objective:
To evaluate oral fluoride (F) retention after using fluoridated toothpastes, rinsing solutions and chewing sticks (Miswaks) in orthodontic patients with and without orthodontic appliances.
Material And Methods:
Nine orthodontic patients, with a mean age of 16 years, were included in a randomized, cross-over, experimental study. Six different home-care F products, two NaF toothpastes (0.32% and 1.1%), two NaF mouthwash solutions (0.05% and 0.2%) and two NaF-impregnated Miswaks chewing sticks (0.05% and 0.5%), were used both during the orthodontic treatment and 1 week after debonding. Unstimulated whole saliva and approximal saliva were collected from two interdental sites, before and up to 60 min after using each product for 2 min. The retention of F was calculated as the area under the 60-min F-clearance curve (AUC).
Results:
In general, the F concentrations at the various sites were higher before than after debonding. Moreover, the products with a high F content (toothpaste, mouthwash and Miswaks) resulted in higher F retention than the corresponding products with a lower F content. In whole saliva, the highest AUC values were found in patients using 0.2% NaF mouthwash, followed by 1.1% NaF toothpaste (p < 0.05). In approximal saliva, the retention values were highest after using 0.5% NaF-impregnated Miswaks in patients wearing orthodontic appliances (p < 0.001).
Conclusions:
The insertion of fixed orthodontic appliances appears to favor oral F retention for all the tested home-care F products. In addition, products with a high F content increase oral F retention.
Related Concept Videos
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...
The Oral Microbiota
