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A Murine Tail Lymphedema Model
04:38

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Published on: February 10, 2021

Surrobodies with functional tails.

Li Xu1, Angeles Estellés, Raffaella Briante

  • 1Sea Lane Biotechnologies, 1455 Adams Drive, Menlo Park, CA 94025, USA.

Journal of Molecular Biology
|January 27, 2010
PubMed
Summary
This summary is machine-generated.

Engineered Surrobodies, based on the pre-B-cell receptor, offer novel protein engineering applications. These unique binding proteins can be functionalized with cytokines or scFv domains for diverse applications.

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Area of Science:

  • Biochemistry
  • Protein Engineering
  • Immunology

Background:

  • Surrobodies are a novel class of binding proteins derived from the pre-B-cell receptor (pre-BCR).
  • Unlike conventional antibodies, pre-BCRs are heterohexameric complexes featuring surrogate light chains (SLCs).
  • The SLCs possess unique non-immunoglobulin-like peptide extensions amenable to protein engineering.

Purpose of the Study:

  • To explore the protein engineering potential of Surrobodies by derivatizing their unique SLC tails.
  • To generate novel Surrobodies with enhanced or bispecific functionalities.
  • To investigate the fusion of cytokines and single-chain variable fragment (scFv) domains to Surrobodies.

Main Methods:

  • Recombinant fusion of fully active cytokines to Surrobodies.
  • Recombinant fusion of single-chain variable fragment (scFv) domains to Surrobodies.
  • Characterization of engineered Surrobodies for novel functions and bispecific binding.

Main Results:

  • Successful generation of Surrobodies with fused cytokines, imparting unique functions.
  • Successful generation of bispecific Surrobodies by fusing scFv domains.
  • Demonstration of Surrobodies as a versatile platform for protein engineering.

Conclusions:

  • Surrobodies represent a unique and adaptable protein scaffold for therapeutic and diagnostic applications.
  • The non-immunoglobulin-like tails of SLCs provide a flexible platform for functional protein fusions.
  • Engineered Surrobodies offer promising avenues for developing novel biologics with tailored specificities and functions.