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Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
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Rapid typing of HLA-DQB1 DNA polymorphism using nonradioactive oligonucleotide probes and amplified DNA

T L Bugawan1, H A Erlich

  • 1Department of Human Genetics, Cetus Corporation, Emeryville, CA 94608.

Immunogenetics
|January 1, 1991
PubMed

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...