Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Incidence and risk factors for surgical site infection after medial opening-wedge high tibial osteotomy using a locking T-shape plate.

International wound journal·2023

Same author

Nanoarchitectonics of nitric oxide releasing supramolecular structures for enhanced antibacterial efficacy under visible light irradiation.

Journal of colloid and interface science·2023

Same author

Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function.

Oxidative medicine and cellular longevity·2023

Same author

Tanshinone IIA-loaded nanoparticles and neural stem cell combination therapy improves gut homeostasis and recovery in a pig ischemic stroke model.

Scientific reports·2023

Same author

Identification of a Novel Keto Sugar Component in Streptococcus pneumoniae Serotype 12F Capsular Polysaccharide and Impact on Vaccine Immunogenicity.

Journal of immunology (Baltimore, Md. : 1950)·2023

Same author

Selectively reflective edge detection system based on cholesteric liquid crystal.

Optics letters·2023

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

Peptide-based probes for targeted molecular imaging.

Seulki Lee¹, Jin Xie, Xiaoyuan Chen

¹Laboratory for Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 31 Center Drive, Suite 1C14, Bethesda, Maryland 20892-2281, USA.

|January 28, 2010

Summary

This summary is machine-generated.

Peptide-based imaging probes offer precise disease diagnosis by targeting specific biomarkers. Advances in phage display and bioconjugation enhance their efficiency for in vivo molecular imaging applications.

More Related Videos

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Development of a ⁶⁸Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Published on: February 3, 2023

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Published on: June 11, 2020

Related Experiment Videos

Last Updated: Jun 16, 2026

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Development of a ⁶⁸Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Published on: February 3, 2023

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Published on: June 11, 2020

Area of Science:

Biomedical Imaging
Molecular Biology
Drug Discovery

Background:

Targeted molecular imaging is crucial for accurate disease diagnosis.
Conventional contrast agents lack specificity and efficiency.
Peptide-based probes are emerging as a successful strategy for targeted imaging.

Purpose of the Study:

To review current trends in peptide-based imaging probes for in vivo applications.
To discuss the advantages and challenges in developing peptide-based probes.
To summarize design strategies and applications of existing peptide-based probe systems.

Main Methods:

Review of current literature on peptide-based imaging probes.
Analysis of phage display and combinatorial peptide chemistry techniques.
Examination of bioconjugation and radiolabeling methods for probe development.

Main Results:

Peptide-based probes demonstrate high targeting efficiency and specificity.
Phage display and combinatorial chemistry have expanded the repertoire of targeting peptides.
Peptides can be readily conjugated with imaging moieties and nanoplatforms.

Conclusions:

Peptide-based probes are highly effective for targeted molecular imaging in vivo.
Continued development in probe design and conjugation techniques is advancing the field.
Peptide-based imaging probes hold significant promise for future diagnostic applications.