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Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
A robust method for quantification of volatile compounds within and between vintages using headspace-solid-phase
Laure Rebière1, Andrew C Clark, Leigh M Schmidtke
1National Wine and Grape Industry Centre, Locked Bag 588, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. lrebiere@csu.edu.au
Analytica Chimica Acta
|January 28, 2010
Summary
A new headspace-solid-phase micro-extraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) method accurately quantifies volatile compounds in Semillon wines. This method ensures reliable data for wine analysis, even across multiple vintages.
Area of Science:
- Analytical Chemistry
- Food Chemistry
- Enology
Background:
- Volatile compounds significantly impact wine aroma and quality.
- Accurate quantification of these compounds is crucial for winemaking and quality control.
- Existing methods may lack the precision or scope for comprehensive volatile profiling.
Purpose of the Study:
- To develop and validate a robust HS-SPME-GC-MS method for quantifying volatile compounds in Hunter Valley Semillon wines.
- To ensure the integrity and reliability of quantitative data, particularly for studies spanning multiple vintages.
- To apply the method to analyze volatile profiles of recent and aged Semillon wines.
Main Methods:
- Utilized a 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane (three-phase) fiber for headspace-solid-phase micro-extraction (HS-SPME).
- Employed four internal standards (methyl isobutyl ketone, n-dodecane, 4-methyl-2-pentanol, ethyl nonanoate) to monitor SPME fiber efficiency and integrity.
- Identified 21 volatile compounds using gas chromatography-mass spectrometry (GC-MS) with two different polarity columns and quantified them using linear regression calibration curves (R² 0.9717–0.9999).
Main Results:
- The developed HS-SPME-GC-MS method demonstrated high linearity and reliability for volatile compound quantification.
- 3-methyl-1-butanol was identified as the most abundant volatile compound in both recent (83 mg L⁻¹) and aged (66 mg L⁻¹) Semillon wines.
- The use of four internal standards effectively monitored and ensured the integrity of the SPME fiber over multiple analyses.
Conclusions:
- The established HS-SPME-GC-MS method provides a reliable approach for the quantitative analysis of volatile compounds in Semillon wines.
- Well-defined procedures, including the use of multiple internal standards, are essential for maintaining data integrity in long-term wine analysis studies.
- This method is applicable to comparing volatile profiles of wines from different vintages, aiding in understanding aging effects.
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