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Updated: Jun 16, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Interaction of guanidine-type surfactants with biological substances
Miyuki Miyake1, Takashi Nishikawa, Masahiko Abe
1Functional Materials Research Laboratory, Lion Corporation, Tokyo, Japan. miyamiyu@lion.co.jp
Abstract:
The interaction of guanidine-type cationic surfactants with bovine serum albumin (BSA) and liposome were investigated. Dodecylguanidine hydrochloride (C(12)A(0)G) and several dodecanoylamide alkylguanidine hydrochlorides (C(12)A(m)G, m = 2, 3, 4, 6) were used as the guanidine-type surfactants. In the interaction of these surfactants with BSA as a model protein, the binding isotherms of the surfactants to BSA were analysed. The structural change of the protein was also examined on the basis of circular dichroism and UV absobance data. In the interaction of these surfactants with liposome as a lipid bilayer model, we studied the effects of the surfactants on the solubilisation of liposomes and the release of carboxyfluorescein from liposomes. In addition, the effect of the surfactant molecular structure on the skin irritation was evaluated in connection with the interactions of the surfactants with BSA and liposome. It was found that small amounts of binding of C(12)A(0)G caused both a partial destruction of a-helix and an aggregation of BSA. C(12)A(0)G also induced the aggregation of liposomes, whereas C(12)A(m)G showed no such action. The presence of A(m) group in C(12)A(m)G appeared to reduce the skin irritation in parallel with the weakening of the interaction of the guanidine group with the protein and the lipid bilayer.
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