Related Experiment Video
Updated: Jun 16, 2026
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
Published on: November 4, 2025
[Fluorescent microbeads-based multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever]
Jian-dong Li1, Shuo Zhang, Quan-fu Zhang
1State Key Laboratory for Infectious Diseases, Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Objective:
To develop and evaluate a multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever.
Methods:
The nucleocapsid proteins (NP) of HTN, SEO, Puu MBV, Lassa, RFV and HPS viruses expressed in prokaryotic cells and purified were coupled to 7 different xMAP fluorescent microbeads. The assay was evaluated and optimized when screened against a panel of reference sera collected from HFRS patients, and compared to commonly used MacELISA Kits.
Results:
For detection of anti-NP antibodies, the sensitivity and specificity of the assay were comparable to a commonly used MacELISA kit, but it could detect different antigen specific antibodies in one reaction simultaneously.
Conclusion:
A robust, rapid and multiplex assay based on NPs could be developed via Luminex xMAP platform for laboratory diagnosis of viral hemorrhagic fever and seroepidemiological investigation.
