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Updated: Jun 16, 2026
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
Published on: February 28, 2019
[Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing
Xin-Chun Chen1, Wei-Long Liu, Gui-Lin Yang
1Shenzhen Third People's Hospital, Shenzhen 518020, China.
Objective:
To generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope.
Methods:
An oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT.
Results:
HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay.
Conclusion:
A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.
