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Updated: Jun 16, 2026
Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
Published on: November 16, 2017
[Study on effect of enzyme linked immunosorbent assay kits of norovirus]
Qing Zhang1, Miao Jin, Shu-Xian Cui
1Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
Objective:
Effects of RIDASCREEN Norovirus (C 1401) 3rd Generation kit (R-biopham AG, darmstadt, Germany) and IDEIA NLV kit (DAKOCytomation., Ely, UK) were compared for detecting human norovirus (HuNV) in fecal sample.
Methods:
The performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction (RT-PCR) by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Guang Zhou. Gene sequencing was performed to positive samples tested by RT-PCR to determine genotype compared with standard sequences.
Results:
RT-PCR is gold standard, RIDASCREEN Norovirus (C 1401) 3rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76%.
Conclusion:
RIDASCREEN Norovirus (C 1401) 3rd Generation kit is more Satisfactory for a preliminary screening.
