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Aging, depot origin, and preadipocyte gene expression.

Mark J Cartwright1, Karen Schlauch, Marc E Lenburg

  • 1Department of Medicine, Boston University, Massachusetts, USA.

The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
|January 29, 2010
PubMed
Summary
This summary is machine-generated.

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Aging alters fat distribution due to changes in fat cell progenitors. Gene expression analysis in rat preadipocytes reveals depot-specific age-related gene expression differences, impacting fat cell function.

Area of Science:

  • Cell Biology
  • Aging Research
  • Metabolic Health

Background:

  • Fat distribution and cell characteristics change with aging.
  • Fat cells (adipocytes) are replaced throughout life, suggesting a role for progenitor cells.
  • Understanding age-related changes in fat cell progenitors is crucial for metabolic health.

Purpose of the Study:

  • To investigate age-related gene expression changes in rat preadipocytes.
  • To compare gene expression profiles between epididymal and perirenal fat depots in young and old rats.
  • To identify specific genes and pathways affected by aging and depot location.

Main Methods:

  • Gene expression profiling of preadipocytes from epididymal and perirenal depots of young and old rats.
  • Analysis of probe sets to identify differentially expressed genes.

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Last Updated: Jun 16, 2026

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

  • Validation of selected genes using polymerase chain reaction (PCR) in preadipocytes from young, middle-aged, and old rats.

    • Main Results:

    • Significant differences in gene expression were observed between fat depots, particularly involving developmental genes.
    • Aging led to fewer significant gene expression changes compared to depot differences, even with relaxed criteria.
    • Thirteen genes showed significant age-related changes, 13 depot-specific changes, and 9 exhibited both.
    • Genes related to inflammation, stress, and differentiation were altered with aging.
    • Age-related changes were more pronounced in perirenal preadipocytes than epididymal ones.

    Conclusions:

    • Age-related changes in preadipocyte gene expression are depot-specific.
    • These depot-specific alterations may contribute to age-related fat redistribution and dysfunction.
    • Understanding these molecular changes can inform strategies for managing age-related metabolic issues.