Systematic chromosomal aberrations found in murine bone marrow-derived mesenchymal stem cells.
Claire Josse1, Renaud Schoemans, Neville-Andrew Niessen
1Department of Human Genetics, Liège University, Liège, Belgium.
Stem Cells and Development
|January 30, 2010
Summary
Murine mesenchymal stem cells (MSCs) exhibit significant chromosomal instability, particularly during senescence. These cells can form tumors, necessitating caution in evaluating their therapeutic potential.
Area of Science:
- Stem cell biology
- Genetics
- Oncology
Background:
- Mesenchymal stem cells (MSCs) are investigated for treating diverse diseases.
- Murine bone marrow-derived MSCs were isolated and cultured for this study.
Purpose of the Study:
- To investigate the chromosomal stability of murine MSCs.
- To determine if MSCs can form tumors.
Main Methods:
- Isolation and cultivation of murine bone marrow-derived MSCs.
- Cytogenetic analysis of MSCs and lymphocytes.
- Observation of tumor formation in mice injected with MSCs.
Main Results:
- Gross chromosomal alterations were observed in MSCs undergoing senescence crisis.
- Lymphocytes from the same animals maintained normal chromosome counts.
- Tumor formation was observed in mice injected with these MSCs.
- Instability was consistent across different mouse strains and culture protocols.
Conclusions:
- Murine MSCs exhibit high chromosomal instability.
- MSCs have tumor-generating potential.
- Caution is advised when using murine MSCs for therapeutic evaluations.
