Related Experiment Video
Updated: Jun 16, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Published on: March 10, 2020
Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
Manuela Marullo1, Chiara Zuccato, Caterina Mariotti
1Department of Pharmacological Sciences and Center for Stem Cell Research, University of Milan, 9 Via Balzaretti, Milan, 20133, Italy. manuela.marullo@unimi.it
Abstract:
We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantification of selected mRNA in the human transcriptome. This result is particularly important for clinical diagnosis and biomarker validation studies based on mRNA detection in human blood.

