Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR01:32

PCR

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stakeholders' perspectives on HIV prevention in the Czech Republic: a qualitative study.

Frontiers in public health·2026
Same author

Bovine Corpus Luteum Proteomics during Different Reproductive and Physiological Stages.

Scientific data·2026
Same author

Extracellular vesicle proteomics in staphylococcus aureus-treated blood and sepsis reveals coordinated complement, acute phase, neutrophil, and exocytosis responses.

Scientific reports·2026
Same author

Author Correction: Regulatory T cells in the mouse hypothalamus control immune activation and ameliorate metabolic impairments in high-calorie environments.

Nature communications·2026
Same author

Effects of Short-term High-dose Vitamin D Supplementation on Mineral Homeostasis in Healthy Young Adults.

In vivo (Athens, Greece)·2026
Same author

Oral paraneoplastic manifestations and findings associated with extra-oral surgically treated malignancies: a prospective cross-sectional study of 300 cases.

World journal of surgical oncology·2026
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

Quality control for quantitative PCR based on amplification compatibility test.

Ales Tichopad1, Tzachi Bar, Ladislav Pecen

  • 1Technical University Munich, Physiology Weihenstephan, Weihenstephaner Berg 3, 85354 Freising-Weihenstephan, Germany. ale@tichopad.de

Methods (San Diego, Calif.)
|January 30, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a new statistical method for quantitative PCR (qPCR) quality control. It accurately identifies inhibited reactions by analyzing amplification data, improving upon existing methods.

More Related Videos

Monochrome Multiplex Quantitative PCR Telomere Length Measurement
11:44

Monochrome Multiplex Quantitative PCR Telomere Length Measurement

Published on: March 22, 2024

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Related Experiment Videos

Last Updated: Jun 16, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

Monochrome Multiplex Quantitative PCR Telomere Length Measurement
11:44

Monochrome Multiplex Quantitative PCR Telomere Length Measurement

Published on: March 22, 2024

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Bioinformatics

Background:

  • Quantitative PCR (qPCR) is vital for nucleic acid quantification but susceptible to errors from inhibition or primer dimers.
  • Current quality control methods often require modifying the sample mix, lacking real-time amplification process monitoring.
  • There is a need for reliable methods to assess qPCR amplification compatibility without altering sample composition.

Purpose of the Study:

  • To develop and validate a novel statistical approach for real-time quality control of qPCR amplification.
  • To assess the reliability of amplification efficiency parameters derived from real-time data.
  • To compare the performance of the proposed multivariate method against traditional univariate approaches.

Main Methods:

  • Utilized multivariate statistical analysis on real-time amplification response data.
  • Fitted the dynamic phase of the amplification trajectory with a suitable model.
  • Calculated Z-score statistics based on model parameters related to amplification efficiency.
  • Compared samples against a predefined reference set of calibration reactions.
  • Employed probabilistic decision-making for Z-score analysis to identify inhibited reactions.

Main Results:

  • The multivariate approach demonstrated improved identification performance for inhibited qPCR reactions compared to univariate methods.
  • The proposed method effectively identified the majority of inhibited reactions in experimental datasets.
  • The study confirmed the utility of amplification efficiency parameters for quality control.

Conclusions:

  • The developed statistical approach offers a reliable method for real-time qPCR quality control without modifying sample mixes.
  • Multivariate analysis of amplification data provides superior performance in detecting reaction incompatibilities.
  • The accuracy of this amplification compatibility test is contingent upon the quality of the reference dataset used.