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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jun 16, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Overlapping splicing regulatory motifs--combinatorial effects on splicing.

Amir Goren¹, Eddo Kim, Maayan Amit

¹Department of Human Genetics and Molecular Medicine, Sackler Faculty of Medicine, Tel-Aviv University, Ramat Aviv 69978, Israel.

Nucleic Acids Research

|January 30, 2010

Summary

Overlapping regulatory sequences in splicing exhibit conserved, functional interplay, influencing gene expression. This evolutionary mechanism, particularly near weak splice sites, enables novel splicing modes not seen with single sites.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Published on: April 22, 2021

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Last Updated: Jun 16, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Published on: September 15, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

Eukaryotic gene splicing relies on numerous splicing factors binding to regulatory sequences on exons and introns.
Combinatorial binding of splicing factors is the presumed mechanism for regulating splicing.
The regulatory role of overlapping splicing regulatory sequences remains largely unexplored.

Purpose of the Study:

To investigate the regulatory role and evolutionary significance of overlapping splicing regulatory sequences.
To determine if overlapping motifs represent a distinct dimension in splicing regulation.

Main Methods:

Computational analysis of experimentally-identified splicing regulatory sequences.
Assessment of nucleotide distribution and sequence overlap patterns.
Experimental validation using a minigene reporter assay.

Main Results:

Identified both positive and negative interplay between overlapping regulatory sequences.
Discovered a unique spatial distribution of overlapping motifs, particularly near weak splice donor sites.
Positively selected overlapping motifs showed high cross-species conservation, indicating functional importance.

Conclusions:

Overlapping splicing regulatory binding sites represent an evolutionarily conserved and widespread mechanism for splicing regulation.
These overlaps can facilitate specific splicing modes not achievable by individual sites.
The study reveals a novel layer of complexity in the combinatorial control of gene splicing.