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Updated: Jun 16, 2026
08:44
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Published on: October 23, 2014
Members of the RSC chromatin-remodeling complex are required for maintaining proper nuclear envelope structure and
Laura C Titus1, T Renee Dawson, Deborah J Rexer
1Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-8240, USA.
Molecular Biology of the Cell
|January 30, 2010
Summary
The RSC chromatin remodeling complex is crucial for nuclear pore complex (NPC) and nuclear envelope (NE) integrity in yeast. Disrupting RSC components leads to NPC mislocalization and altered NE structure.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Nuclear pore complexes (NPCs) and the nuclear envelope (NE) are vital for nuclear architecture.
- Mechanisms governing NPC and NE structure and function are not fully understood.
Purpose of the Study:
- To identify genes regulating nuclear pore complex (NPC) localization and nuclear envelope (NE) integrity in Saccharomyces cerevisiae.
- To elucidate the role of the RSC chromatin remodeling complex in NPC and NE structure.
Main Methods:
- Utilized two genetic screening approaches in yeast (Saccharomyces cerevisiae) to identify mutants with defects in NPC localization.
- Examined GFP-Nic96 localization under depleted essential genes using a Tet-promoter system.
- Characterized a temperature-sensitive mutant (sth1-F793S) and employed electron microscopy and benzyl alcohol treatment.
Main Results:
- Depletion of RSC chromatin remodeling complex components caused significant NPC mislocalization.
- NPC mislocalization required active transcription and protein synthesis.
- Altered NE structure, nuclear morphology, and cytoplasmic membrane accumulation were observed; benzyl alcohol treatment ameliorated defects.
Conclusions:
- The RSC chromatin remodeling complex plays a critical role in maintaining nuclear pore complex (NPC) and nuclear envelope (NE) structural integrity.
- Nuclear envelope structure is functionally linked to chromatin architecture, suggesting a coordinated regulation.
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