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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
Published on: April 5, 2018
Modern approaches for investigating epigenetic signaling pathways
Adam G Evertts1, Barry M Zee, Benjamin A Garcia
1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Journal of Applied Physiology (Bethesda, Md. : 1985)
|January 30, 2010
Summary
Epigenetics, involving DNA methylation and histone modifications, regulates gene expression in physiological processes like obesity. This review highlights techniques for studying these epigenetic marks quantitatively across conditions.
Area of Science:
- Physiological processes
- Molecular biology
- Genomics
Background:
- Epigenetics, through DNA methylation and histone modifications, influences gene transcription without altering DNA sequence.
- Epigenetic mechanisms are crucial for physiological processes such as obesity and circadian rhythms.
- Quantitative analysis of epigenetic regulation is essential for understanding its role in health and disease.
Purpose of the Study:
- To review the latest techniques for identifying and comparing novel DNA methylation and histone modification patterns.
- To describe methods for quantitatively assessing epigenetic regulation in physiological processes.
- To outline strategies for localizing epigenetic marks to specific genomic regions.
Main Methods:
- Review of current methodologies in epigenetics research.
- Focus on techniques for analyzing DNA methylation and histone posttranslational modifications.
- Discussion of methods for comparative and localization studies of epigenetic marks.
Main Results:
- Identification of advanced techniques for epigenetic analysis.
- Emphasis on quantitative comparison of epigenetic marks across diverse experimental settings.
- Methods for precise genomic localization of DNA methylation and histone modifications.
Conclusions:
- Advanced techniques enable more quantitative insights into epigenetic regulation.
- Understanding epigenetic modifications is key to unraveling complex physiological processes.
- This review provides a resource for researchers studying the molecular basis of epigenetics.
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01:46Epigenetic Regulation
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