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Updated: Jun 16, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Diffraction-limited holography.

R J Bieringer, J A Ringlien

    Applied Optics
    |January 30, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Holographic image reconstruction achieved Airy disk sizes comparable to diffraction limits. Photographic tests showed slight discrepancies attributed to film contrast enhancement, not fundamental holographic limitations.

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    Area of Science:

    • Optics and Photonics
    • Holography
    • Image Reconstruction

    Background:

    • Holograms capture wavefronts, enabling 3D image reconstruction.
    • Diffraction limits define the theoretical resolution of optical systems.
    • Real image reconstructions from limited holograms require careful analysis.

    Purpose of the Study:

    • To experimentally determine the Airy disk size in real holographic image reconstructions.
    • To compare experimental results with theoretical diffraction limitations.
    • To investigate discrepancies between photographic and photoelectric measurement methods.

    Main Methods:

    • Transmission, side-band holograms of ~3-microm objects were created.
    • Real images were reconstructed with unit magnification and viewed at 400x.
    • Comparisons used visual, photographic (bar patterns), and photoelectric recordings.

    Main Results:

    • Experimental Airy disk sizes were comparable to diffraction-limited values.
    • Photographic resolution tests yielded an Airy disk radius ratio of 1.05.
    • Discrepancies between photographic and photoelectric measurements were observed.

    Conclusions:

    • Holographic real image reconstruction approaches diffraction-limited resolution.
    • Film contrast enhancement can influence photographic resolution measurements.
    • Photoelectric measurements may offer a more direct assessment of holographic resolution.