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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...

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Related Experiment Video

Updated: Jun 16, 2026

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

Wnt gene expression in human trabecular meshwork cells.

Rajalekshmy Shyam1, Xiang Shen, Beatrice Y J T Yue

  • 1Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago College of Medicine, Chicago, IL, USA.

Molecular Vision
|January 30, 2010
PubMed
Summary
This summary is machine-generated.

Human trabecular meshwork (TM) cells express Wnt signaling genes. Oxidative stress minimally impacts Wnt activity in TM cells, suggesting its limited role in glaucoma pathogenesis.

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Last Updated: Jun 16, 2026

Studying Wnt Signaling During Patterning of Conducting Airways
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Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Cell Biology

Background:

  • The Wnt signaling pathway regulates crucial cellular processes.
  • Trabecular meshwork (TM) cells are vital for maintaining intraocular pressure.
  • Dysregulation of TM cell function is implicated in glaucoma development.

Purpose of the Study:

  • To investigate Wnt signaling gene expression in human TM cells.
  • To assess the impact of oxidative stress on Wnt signaling in TM cells.
  • To identify specific Wnt pathway components, including beta-catenin (CTNNB1) and secreted frizzled-related protein-1 (sFRP1).

Main Methods:

  • Human TM cells were cultured and subjected to oxidative stress using hydrogen peroxide (H2O2).
  • Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect Wnt gene expression.
  • Western blotting was utilized to quantify Wnt-related protein levels post-treatment.

Main Results:

  • A comprehensive set of 25 Wnt and Wnt-related genes were detected in human TM cells.
  • The majority of identified genes were associated with the Wnt/beta-catenin pathway, with noncanonical pathway members also present.
  • Oxidative stress did not induce significant alterations in beta-catenin and sFRP1 protein levels.

Conclusions:

  • Human TM cells express genes involved in both canonical and noncanonical Wnt signaling pathways.
  • All three major Wnt pathways appear to be functional within the TM system.
  • Despite its role in glaucoma, oxidative stress exhibited minimal influence on Wnt pathway activity in TM cells.