Related Experiment Video
Updated: Jun 16, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
Published on: September 20, 2016
Chemistry and biological applications of photo-labile organic molecules
Haitao Yu1, Jinbo Li, Dongdong Wu
1School of Chemistry and Chemical Engineering, Key Lab of Analytical Chemistry for Life Science, Ministry of Education of China, Nanjing University, Nanjing, 210093, PR China.
Abstract:
Photo-labile molecules have been widely used not only in organic synthesis but also in biological study. The chemistry of the typical photo-labile organic molecules, including their structure, mechanism underlying their photo-lability and strategies to integrate them with biomolecules, is reviewed to illustrate the structural basis for photo-activable caged compounds. Biological applications of representative photo-labile caged molecules were also illustrated for a general understanding on the important roles of caged compounds in dynamic biological studies. This tutorial review would provide an interdisciplinary overview on the important area of chemical biological study making use of photo-labile caged compounds.
More Related Videos
Related Concept Videos
Photoluminescence: Applications
Applications of IR Spectroscopy: Overview
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Variables Affecting Phosphorescence and Fluorescence
Cycloaddition Reactions: MO Requirements for Photochemical Activation
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

