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[Cloning and identification of the plasmid replication gene repC in Mesorhizobium huakuii]

Guoyuan Hu1, Shanming Wang, Weiwei Li

  • 1Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan 430073, China. hgy701@tom.com

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|February 2, 2010
PubMed
Abstract

Insights

Researchers identified repC-like sequences in Mesorhizobium huakuii, crucial for plasmid replication. These sequences showed high similarity within the strain but differed from other rhizobia, aiding in plasmid research.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Context:

  • The repC gene is essential for initiating plasmid replication in bacteria.
  • Mesorhizobium huakuii strain HN3015 and its plasmid-cured derivatives were used for this study.

Purpose:

  • To identify and characterize repC-like sequences in Mesorhizobium huakuii strain HN3015.
  • To determine the location and sequence homology of these repC-like sequences.

Summary:

  • RepC-like sequences were successfully amplified and sequenced from Mesorhizobium huakuii using polymerase chain reaction (PCR) with RC1 and RC3 primers.
  • Southern blotting confirmed that these sequences are plasmid-associated. The amplified products were approximately 750 bp.
  • Sequence analysis revealed 100% similarity among the repC sequences within the tested strains, yet significant differences compared to repC genes from other rhizobia.

Impact:

  • This study provides a detailed characterization of the repC gene in Mesorhizobium huakuii.
  • The findings contribute to understanding plasmid replication mechanisms in rhizobia.
  • The identified sequences can serve as markers for plasmid research in related bacterial species.

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