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Updated: Jun 16, 2026

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
Published on: January 21, 2019
High contrast, depth-resolved thermoreflectance imaging using a Nipkow disk confocal microscope
J A Summers1, T Yang, M T Tuominen
1Department of Physics, Mount Holyoke College, South Hadley, Massachusetts 01075, USA.
Abstract:
We have developed a depth-resolved confocal thermal imaging technique that is capable of measuring the temperature distribution of an encapsulated or semi-obstructed device. The technique employs lock-in charge coupled device-based thermoreflectance imaging via a Nipkow disk confocal microscope, which is used to eliminate extraneous reflections from above or below the imaging plane. We use the confocal microscope to predict the decrease in contrast and dynamic range due to an obstruction for widefield thermoreflectance, and we demonstrate the ability of confocal thermoreflectance to maintain a high contrast and thermal sensitivity in the presence of large reflecting obstructions in the optical path.
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