Hypercapnic acidosis reduces contractile function in the ventricle of the armored catfish, Pterygoplichthys pardalis

The armored catfish, Pterygoplichthys pardalis (formerly Liposarcus pardalis), is a freshwater, facultative air-breathing teleost that experiences seasonal hypercapnia in the water systems of South America. We studied the tolerance of the P. pardalis heart to hypercapnic acidosis using an isolated ventricular muscle strip preparation. Force generation and kinetic variables were examined across a range of contraction frequencies under normocapnic and hypercapnic conditions in the absence and presence of sarcoplasmic reticulum (SR) inhibitors. Pterygoplichthys pardalis ventricle exhibited robust contractile force, on par with athletic fish species such as trout and tuna and a relatively flat force-frequency relationship between 0.2 and 1.5 Hz under normocapnic conditions (1% CO2, pH 7.78 +/- 0.02). Hypercapnic acidosis (7.5% CO2, pH 7.78 +/- 0.03) did not alter the shape of the force-frequency response but reduced force by approximately 50% across all frequencies tested, with only partial recovery upon return to normocapnic conditions. A subsequent and more severe acidotic challenge (15% CO2, pH 6.77 +/- 0.05) caused an additional 20% decrease in force. Force recovered to the level at which it had stablized after the first hypercapnic insult. SR inhibition had no steady state effect on force production at 0.2 Hz but resulted in a negative force-frequency relationship, suggesting that SR Ca2+ is recruited to a greater extent at high contraction frequencies. Surprisingly, SR-inhibited muscle was more resistant to hypercapnic acidosis (force decreased by approximately 40% across all frequencies) and displayed improved recovery upon return to normocapnic conditions. The significance of this latter finding is not clear. In aggregate, our results demonstrate robust contractile force, which extends across a range of frequencies and appears to be supported by SR Ca2+ cycling. Hypercapnic acidosis reduced contractile force but may provide preconditioning-like protection from subsequent insults.