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LED-Based In Vitro Screening for Assessing Photoactivable Molecules in Bacterial Photodynamic Inactivation
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Nanoparticle-based endodontic antimicrobial photodynamic therapy.

Tom C Pagonis1, Judy Chen, Carla Raquel Fontana

  • 1Advanced Graduate Endodontics, Division of Endodontics, Harvard School of Dental Medicine, Boston, Massachusetts, USA.

Journal of Endodontics
|February 2, 2010
PubMed
Summary
This summary is machine-generated.

Poly(lactic-co-glycolic acid) nanoparticles loaded with methylene blue and light show antimicrobial effects against Enterococcus faecalis. This combination significantly reduced bacterial levels in planktonic cultures and infected root canals.

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Area of Science:

  • Biomaterials Science
  • Microbiology
  • Nanotechnology

Background:

  • Enterococcus faecalis is a common pathogen in endodontic infections.
  • Photodynamic therapy offers a potential antimicrobial strategy.
  • Nanoparticle drug delivery systems can enhance therapeutic efficacy.

Purpose of the Study:

  • To evaluate the in vitro antimicrobial efficacy of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with methylene blue (MB) combined with red light.
  • To investigate the uptake and distribution of MB-loaded PLGA nanoparticles in Enterococcus faecalis.

Main Methods:

  • Transmission electron microscopy (TEM) was used to visualize nanoparticle uptake by E. faecalis.
  • E. faecalis in planktonic suspension and infected root canals were treated with MB-loaded PLGA nanoparticles and red light (665 nm).
  • Bacterial viability was assessed by colony-forming unit (CFU) counts.

Main Results:

  • Nanoparticles primarily localized on the cell walls of E. faecalis.
  • A synergistic effect of MB-loaded nanoparticles and light resulted in significant bacterial reduction.
  • Approximately 2 log10 CFU reduction in planktonic phase and 1 log10 CFU reduction in root canals were observed.

Conclusions:

  • PLGA nanoparticles loaded with photosensitizers like MB show promise as an adjunct in endodontic antimicrobial therapy.
  • The combination of nanoparticles, photosensitizer, and light demonstrates potent antimicrobial activity.
  • Further research into nanoparticle-based photodynamic therapy for endodontics is warranted.