[Spectrofluorometric ultramicroassay for determining the plasma lipid peroxidation index by the thiobarbituric acid...

A simple, inexpensive spectrofluorometric ultramicroassay for determining the plasma lipid peroxidation index is described. Plasma is incubated for 60 minutes at 95 degrees C in a thiobarbituric acetate buffer medium (pH 3,5) with butylhydroxytoluene and Fe . The chromogen is then extracted in butanol 1-pyridine and quantified by spectrofluorometry (exc.: 532 nm; em: 553 nm). The mean value of the plasma thiobarbituric lipid peroxidation index determined by this method in 101 healthy non-smokers (age 39.1 +/- 8.7 years) was found to be 1.61 +/- 0.72.