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Related Experiment Videos

Multiple Oct2 isoforms are generated by alternative splicing.

T Wirth1, A Priess, A Annweiler

  • 1Zentrum für Molekulare Biologie, Universität Heidelberg (ZMBH), FRG.

Nucleic Acids Research
|January 11, 1991
PubMed
Summary
This summary is machine-generated.

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Mouse Oct2 transcription factor isoforms are generated by alternative splicing and can activate gene expression in non-lymphoid cells, revealing functional redundancy in B-cell regulation.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Immunology

Background:

  • The Oct2 transcription factor regulates lymphoid-specific immunoglobulin gene expression through binding to the octamer motif.
  • Ectopic Oct2 expression can restore some lymphoid-specific gene regulation in non-lymphoid cells.

Purpose of the Study:

  • To isolate and characterize mouse Oct2 cDNAs from a mature B-cell line.
  • To investigate the generation and function of Oct2 isoforms in gene regulation.

Main Methods:

  • cDNA isolation and characterization from a mature B-cell line.
  • Alternative splicing analysis.
  • Cotransfection experiments in fibroblasts.

Main Results:

  • Multiple Oct2 isoforms are generated from a single gene via alternative splicing.

Related Experiment Videos

  • All isoforms possess a functional POU domain, enabling octamer motif binding.
  • Isoforms are present in varying amounts within B-cells and are conserved between mice and humans.
  • All isoforms activate octamer-containing promoters in fibroblasts, indicating functional redundancy.

    • Conclusions:

    • Alternative splicing generates diverse Oct2 isoforms with conserved DNA-binding capacity.
    • Functional redundancy exists among Oct2 isoforms in activating gene expression.
    • One identified isoform corresponds to the lymphoid-specific Oct2B protein, implicated in immunoglobulin heavy-chain enhancer function.