Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mapping sequenced E.coli genes by computer: software, strategies and examples.

K E Rudd1, W Miller, C Werner

  • 1Laboratory of Bacterial Toxins, Food and Drug Administration, Bethesda, MD 20892.

Nucleic Acids Research
|February 11, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impacts of droughts and drought legacies on plants and ecosystems.

Plant biology (Stuttgart, Germany)·2026
Same author

Treadmill perturbation-based balance training to prevent unrecovered falls in fall-prone older adults with and without cognitive impairment: protocol for the multi-center randomized controlled TRAIL study.

BMC geriatrics·2025
Same author

[Geriatrie rehabilitation in different settings].

Zeitschrift fur Gerontologie und Geriatrie·2025
Same author

Impact of emerging compound droughts on forests: A water supply and demand perspective.

Plant biology (Stuttgart, Germany)·2025
Same author

No legacy effects of severe drought on carbon and water fluxes in a Mediterranean oak forest.

Plant biology (Stuttgart, Germany)·2025
Same author

Der Nervenarzt·2025
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
Same journal

Evolution of the ribosomal exit tunnel through the eyes of the nascent chain.

Nucleic acids research·2026
Same journal

Enhancing the performance and interpretability of epigenetic clocks.

Nucleic acids research·2026
Same journal

FABIAN-variant 2026: improved prediction of the effects of DNA variants on transcription factor binding.

Nucleic acids research·2026
Same journal

Structural and biochemical characterization of Grimontia hollisae thermostable direct hemolysin with DNA reveals first Vibrio hemolysin with nuclease activity.

Nucleic acids research·2026
See all related articles

This study presents novel software and strategies for organizing diverse DNA data, including sequence and genetic maps, enabling the analysis of large genomic regions like E. coli. The methods ensure data accuracy and are applicable to various genome sequencing projects.

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Integrating diverse genomic data (DNA sequences, restriction maps, genetic maps) from multiple sources is challenging.
  • Existing tools may not adequately handle the complexity and heterogeneity of biological data.
  • Accurate genome organization is crucial for large-scale sequencing efforts.

Purpose of the Study:

  • To describe methods and novel software for organizing and integrating disparate DNA data.
  • To present strategies for resolving data inconsistencies and ensuring accuracy.
  • To demonstrate the application of these methods in analyzing over one megabase of E. coli DNA sequence.

Main Methods:

  • Development of a suite of novel software tools for data organization and integration.

Related Experiment Videos

  • Application of specific strategies to manage and resolve data inconsistencies.
  • Utilizing high-resolution restriction maps for genome analysis.

    • Main Results:

    • Successfully ordered, analyzed, and displayed over one megabase of E. coli DNA sequence data.
    • Demonstrated the effectiveness of software and strategies in handling diverse and sometimes conflicting data.
    • Exposed data inconsistencies, highlighting the need for biological judgment in curation.

    Conclusions:

    • The described software and mapping methods facilitate the integration of diverse genomic data.
    • These approaches are valuable for both weakly and highly coordinated genome sequencing projects.
    • Effective data integration requires a combination of automated tools and expert biological insight.