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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Performance of columns packed with the new shell particles, Kinetex-C18
Fabrice Gritti1, Irene Leonardis, David Shock
1Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
Journal of Chromatography. A
|February 2, 2010
Summary
The new Kinetex-C18 column, packed with porous shell particles, demonstrates superior chromatographic efficiency for small molecules and proteins. Its performance surpasses previous records, offering faster mass transfer and higher resolution in analytical separations.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- High-performance liquid chromatography (HPLC) relies on column efficiency for separation quality.
- Porous shell particles offer potential advantages in mass transfer kinetics and efficiency.
Purpose of the Study:
- To evaluate the chromatographic performance of the novel Kinetex-C18 column.
- To compare its efficiency and mass transfer characteristics against established columns.
Main Methods:
- Investigated column efficiency using low molecular weight compounds (e.g., anthracene, naphtho[2,3-a]pyrene).
- Assessed liquid-solid mass transfer for proteins (e.g., insulin, lysozyme).
- Corrected efficiency data for instrument extra-column volume contributions.
Main Results:
- Achieved minimum reduced plate heights between 1.0 and 1.3 for small molecules, surpassing previous benchmarks.
- Demonstrated exceptionally fast liquid-solid mass transfer for proteins, significantly outperforming Halo-C18 columns.
- Quantified contributions of dispersion and mass transfer resistances to overall column efficiency.
Conclusions:
- The Kinetex-C18 column exhibits outstanding chromatographic efficiency, setting new performance standards.
- Its rapid mass transfer kinetics make it highly suitable for protein analysis.
- The design utilizing porous shell particles is a key factor in its superior performance.
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