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Updated: Jun 16, 2026

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices
Published on: October 3, 2016
[Knowledge and adherence to bio-safety measures and biological accidents by nursing students during their clinical
Felicitas Merino-de la Hoz1, María Jesús Durá-Ros, Elías Rodríguez-Martín
1Departamento de Enfermería, Universidad de Cantabria, Santander, España. merinof@unican.es
Objective:
To identify the degree of knowledge and performance of bio-safety measures by nursing students and knowing the type of biological accidents suffered during their clinical practice.
Method:
A cross-sectional study was conducted on the students of three Nursing courses held in May of 2008. Data was collected by an anonymous self-administered questionnaire, with a return of 54%.
Results:
A total of 97% of students seemed to know the standard biosafety measures, and all of them (100%) stated that those measures must be applied to every patient. However, the reality of clinical practice shows that biosafety measures are only partially applied. An average of 60.2% implement the personal hygiene measures, 66.1% use physical barriers, and 44% use sharp materials safely. Around 32.25% of the students have suffered some biological accident, with a greater incidence in the second year: administering injections (24%), drawing blood samples with Venojet needles (18%) and recapping used needles (17%).
Conclusions:
The high level of knowledge shown by the students on standard precautions is not always shown in clinical practice. There are significant deficiencies in student safety practices: recapping of used needles continues to be one of the most common risk practices carried out.
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