Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Cell Signaling Feedback Loops01:07

Cell Signaling Feedback Loops

Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diverse mechanisms of translation arrest by a Clostridia ribosome stalling peptide CliM.

Nature communications·2026
Same author

Structural basis for DNA processing and membrane translocation by ComEC in natural transformation.

Science (New York, N.Y.)·2026
Same author

Evolutionary adaptation of bacterial proteomes to translation-impeding sequences.

The EMBO journal·2025
Same author

Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.

Cell host & microbe·2025
Same author

Developmentally regulated proteolysis by MdfA and ClpCP mediates metabolic differentiation during <i>Bacillus subtilis</i> sporulation.

Genes & development·2025
Same author

Deciphering metabolic differentiation during Bacillus subtilis sporulation.

Nature communications·2025
Same journal

Quercetin suppresses TGF-β1-induced proliferation and migration of vascular smooth muscle cells via the Smad2/3/MMP-9 signaling axis.

Biochemical and biophysical research communications·2026
Same journal

Biosynthesis, characterization and biological potential of microbe-mediated silver nanoparticles using thermophilic actinomycetes, Streptomyces nigra.

Biochemical and biophysical research communications·2026
Same journal

COP9 signalosome 8 mediated autophagy drives proliferation, invasion, and metastasis in pancreatic ductal adenocarcinoma.

Biochemical and biophysical research communications·2026
Same journal

Tumor budding in colorectal cancer: partial EMT, microenvironmental remodeling, and metastatic competence.

Biochemical and biophysical research communications·2026
Same journal

Exploring the therapeutic versatility and multitarget pharmacological potential of acyl hydrazone-hydrazide scaffolds.

Biochemical and biophysical research communications·2026
Same journal

The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

Biochemical and biophysical research communications·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

Divergent stalling sequences sense and control cellular physiology.

Koreaki Ito1, Shinobu Chiba, Kit Pogliano

  • 1Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603-8555, Japan. kito@cc.kyoto-su.ac.jp

Biochemical and Biophysical Research Communications
|February 2, 2010
PubMed
Summary
This summary is machine-generated.

Certain amino acid sequences can halt ribosome activity, impacting gene expression. This ribosome stalling is influenced by compounds or membrane interactions and affects messenger RNA structure.

More Related Videos

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
09:50

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Published on: August 27, 2015

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
08:06

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Published on: March 19, 2021

Related Experiment Videos

Last Updated: Jun 16, 2026

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
09:50

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Published on: August 27, 2015

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
08:06

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Published on: March 19, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Recent research has uncovered specific amino acid sequences capable of interacting with ribosomal components.
  • These sequences can induce ribosome stalling, arresting protein synthesis elongation.
  • Ribosome stalling has been observed to influence gene expression regulation.

Purpose of the Study:

  • To investigate the mechanisms and implications of amino acid sequence-induced ribosome stalling.
  • To differentiate between inducible and intrinsic classes of ribosome stalling.
  • To understand how ribosome stalling affects messenger RNA secondary structure and gene expression.

Main Methods:

  • Analysis of amino acid sequences known to interact with ribosomal interiors.
  • Characterization of factors influencing inducible (low-molecular weight compounds) and intrinsic (membrane interaction) stalling.
  • Examination of messenger RNA secondary structure alterations in response to ribosome stalling.
  • Investigation of the regulatory impact on cis-located target gene expression.

Main Results:

  • Identified distinct amino acid sequences that cause ribosome elongation arrest.
  • Differentiated stalling mechanisms: inducible class requires compounds, intrinsic class is released by membrane transport/insertion.
  • Demonstrated that stalled ribosomes alter messenger RNA secondary structure.
  • Showed that ribosome stalling regulates cis-located target gene expression at multiple levels.

Conclusions:

  • Amino acid sequences can directly regulate gene expression through ribosome stalling.
  • Stalling mechanisms are diverse, involving specific compounds or membrane interactions.
  • Ribosome stalling represents a novel regulatory mechanism impacting gene expression via messenger RNA structural changes.