Single domain antibody templated nanoparticle resistors for sensing

Area of Science:

• Biomolecular engineering
• Nanoparticle synthesis
• Biosensor development

Background:

• Biomolecules offer versatile platforms for ambient condition material synthesis, enabling precise control over nanoparticle characteristics.
• Biomolecules can confer recognition and sensing abilities to metal nanoparticles, crucial for diagnostic applications.
• Cholera toxin poses a significant public health threat, necessitating sensitive and rapid detection methods.

Purpose of the Study:

• To utilize llama single domain antibodies for templating gold nanoparticle synthesis.
• To assemble antibody-functionalized gold nanoparticles onto electrodes for biosensing.
• To develop a sensitive detection method for cholera toxin using these nanoparticle assemblies.

Main Methods:

• Selection of llama single domain antibodies targeting the beta-subunit of cholera toxin.
• Templated synthesis of gold nanoparticles using the selected antibodies.
• Assembly of antibody-coated gold nanoparticles onto electrodes via dielectrophoresis.
• Detection of cholera toxin by monitoring changes in the conductance of the antibody-coated gold nanoparticle assemblies.

Main Results:

• Successful templated synthesis of gold nanoparticles guided by llama single domain antibodies.
• Formation of functional antibody-coated gold nanoparticle assemblies on electrodes.
• Demonstrated detection of cholera toxin through measurable changes in electrical conductance.

Conclusions:

• Llama single domain antibodies can effectively template gold nanoparticle synthesis for biosensor applications.
• Dielectrophoretic assembly provides a viable method for creating nanoparticle-based biosensors.
• The developed biosensor shows promise for sensitive and specific detection of cholera toxin.