Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Programmed demethylation in CpG islands during human fetal development.

B R Migeon1, M M Holland, D J Driscoll

  • 1Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Somatic Cell and Molecular Genetics
|March 1, 1991
PubMed
Summary

DNA methylation patterns in adult mammals are established during fetal development. Specific demethylation events during weeks 6-14 of gestation program these adult DNA methylation patterns for housekeeping genes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The presentation, course and outcome of COVID-19 infection in people with Prader-Willi syndrome: unexpected findings from an international survey.

Orphanet journal of rare diseases·2022
Same author

Skin-Related complications of Klippel-Trenaunay Syndrome: a retrospective review of 410 patients.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

EVALUATION OF PLASMA SUBSTANCE P AND BETA-ENDORPHIN LEVELS IN CHILDREN WITH PRADER-WILLI SYNDROME.

The Journal of rare disorders·2016
Same author

A reduced-energy intake, well-balanced diet improves weight control in children with Prader-Willi syndrome.

Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2012
Same author

Epigenetic regulation of pericentromeric heterochromatin during mammalian meiosis.

Cytogenetic and genome research·2010
Same author

Identification of association of common AGGF1 variants with susceptibility for Klippel-Trenaunay syndrome using the structure association program.

Annals of human genetics·2008

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Genomics

Background:

  • The establishment of DNA methylation patterns in adult mammalian tissues remains poorly understood.
  • Investigating the timing of DNA methylation pattern establishment for housekeeping genes is crucial for understanding developmental processes.

Purpose of the Study:

  • To determine when adult DNA methylation patterns are established for specific housekeeping genes.
  • To elucidate the role of demethylation in programming these patterns during fetal development.

Main Methods:

  • Analysis of clustered CpGs in active X chromosome genes (PGK, G6PD, P3, GdX, HPRT) and the autosomal gene DHFR.
  • Examination of DNA methylation patterns in fetal and adult mammalian tissue specimens.

Related Experiment Videos

Main Results:

  • Unique DNA methylation patterns were observed at the P3 locus in all tissues from 6- to 9-week fetal specimens.
  • Distinct methylation patterns were found at the HPRT locus in adrenal gland DNA at the same developmental stage.
  • Adult DNA methylation patterns are established post-fetally through the demethylation of specific CpGs.

Conclusions:

  • Demethylating events targeting CpG islands are programmed during mammalian fetal development.
  • De novo methylation in the fetus may occur at the 3' region of CpG islands in active genes.
  • Sequence-specific demethylation during 6-14 weeks of fetal development establishes adult DNA methylation patterns.